04 September 2020>: Lab/In Vitro Research
Icariin Protects Mouse Insulinoma Min6 Cell Function by Activating the PI3K/AKT Pathway
Tao Zhang 1ABCDEFG , Fen Qiu 2BCDEFG*DOI: 10.12659/MSM.924453
Med Sci Monit 2020; 26:e924453
Figure 2 Icariin reversed the effects of UA on Min6 cell viability and apoptosis. Min6 cells were treated with various concentration of icariin (0, 5, 10, 20 μM) for 2 h, followed by treatment with 5 mg/dl UA for another 24 h. (A) Cell viability and (B) apoptosis were assessed using MTT and flow cytometry analysis, respectively. (C) Quantity of apoptotic cells. (D) Protein levels of cleaved caspase-3 and pro-caspase-3 were examined using western blotting. ** P<0.01 compared with the control group; # P<0.05, ## P <0.01 compared with the UA group.