Figure 5 LY294002 reversed the protective effect of icariin in UA-induced Min6 cell injury. Min6 cells were treated with LY294002, UA and/or icariin for the indicated times. Cells were divided into two groups: UA+20 μM icariin and UA+20 μM icariin+LY294002. (A) Cell viability and (B) apoptosis were assessed using MTT and flow cytometry analysis, respectively. (C) Cell apoptosis. (D) Protein levels of cleaved caspase-3 and pro-caspase-3 measured by western blotting. (E) Secretion of insulin quantified by GSIS. ** P<0.01 compared with the control group; # P<0.05, ## P<0.01 compared with the UA group.