04 September 2020>: Lab/In Vitro Research
Icariin Protects Mouse Insulinoma Min6 Cell Function by Activating the PI3K/AKT Pathway
Tao Zhang 1ABCDEFG , Fen Qiu 2BCDEFG*DOI: 10.12659/MSM.924453
Med Sci Monit 2020; 26:e924453
Figure 6 LY294002 reversed the effect of icariin on the PI3K/AKT pathway in UA-treated Min6 cells. Min6 cells were treated with LY294002, UA and/or icariin for the indicated times. Cells were divided into two groups: UA+20 μM icariin and UA+20 μM icariin+LY294002. (A) Expression of P-PI3K and p-AKT proteins by western blotting. (B) p-PI3K/PI3K and (C) p-AKT/AKT ratios. Levels of (D) PI3K and (E) AKT mRNAs determined by qRT-PCR. ** P<0.01 compared with the UA+20 μM icariin group.