22 August 2020 : Animal Research
Role of Sirtuin-1 in Neonatal Hypoxic-Ischemic Encephalopathy and Its Underlying Mechanism
Zhen Zhang1ABCDEFG, Xin Chen1ABCDEFG, Sichen Liu2BCEFG*DOI: 10.12659/MSM.924544
Med Sci Monit 2020; 26:e924544
Figure 1 SIRT1 expression was downregulated in the HIE rat brain tissues. (A) Cell apoptosis of rat brain tissues was analyzed using flow cytometry. (B) Cell apoptosis rate was calculated and presented (n=5). (C) The levels of the related molecular markers S100B and NSE during the development of HIE were measured by Western blot. (D) Western blot analysis of SIRT1 levels. (E) qRT-PCR analysis of SIRT1 mRNA levels (n=5). The results showed as the mean ± SD. ** P<0.01 vs. control group. SIRT1 – sirtuin-1; HIE – hypoxic-ischemic encephalopathy; NSE – neuron-specific enolase; S100B – S100 calcium binding protein B.