Figure 3 Transfection efficiency of SIRT1 overexpression (SIRT1-plasmid) or silencing (SIRT1-siRNA) in primary rat neuronal cells. SIRT1 protein levels and mRNA gene expression in primary rat neuronal cells transfected with SIRT1-plasmid or control-plasmid for 48 h were determined by (A) Western blot and (B) qRT-PCR assays, respectively. SIRT1 protein levels and mRNA gene expression in primary rat neuronal cells transfected with SIRT1-siRNA or control-siRNA for 48 h were determined by (C) Western blot and (D) qRT-PCR assays, respectively. Control – cells without any treatment; Control-plasmid: cells were transfected with control-plasmid; SIRT1-plasmid: cells were transfected with SIRT1-plasmid; Control-siRNA: cells were transfected with control-siRNA; SIRT1-siRNA: cells were transfected with SIRT1-siRNA. The results showed as the mean±SD. ** P<0.01 vs. control group. SIRT1 – sirtuin-1.