Figure 4 PAK2 silencing reversed the inhibitive effect of METRNL overexpression on inflammatory response. (A) The expression of PAK2 in H9C2 cells was determined by western blot. (B) Relative expression of PAK2 in H9C2 cells was evaluated by qRT-PCR. (C) The levels of inflammatory cytokines including TNF-α, IL-1β, and IL-6 were measured by corresponding ELISA kits. Error bars represent the mean±SEM from three independent experiments. *** P<0.001 versus Control; ### P<0.001 versus OGD/R 12 hours; @@ P<0.01, @@@ P<0.001 versus OGD/R+Ov-METRNL. PAK2 – p21-activated kinase 2; METRNL – Meteorin-like; qRT-PCR – quantitative real-time polymerase chain reaction; TNF – tumor necrosis factor; IL – interleukin; ELISA – enzyme-linked immunosorbent assay; SEM – standard error of the mean; OGD/R – oxygen-glucose deprivation and reperfusion.