07 September 2020: Animal Study
A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors
Cheng Zhou 1BCDEF , Chuan Ye 2BCDF , Chen Zhao 1BCD , Junyi Liao 1BCF , Yuwan Li 1BC , Hong Chen 1BF , Wei Huang 1ADEG*DOI: 10.12659/MSM.924666
Med Sci Monit 2020; 26:e924666
Figure 4 The LV-BMP9 infection enhanced cell viability and upregulated mRNA transcriptions of osteogenic factors (BMP9, RUNX2, OCN, OPN, OSX) in BMSCs. (A) Determination of LV or LV-BMP9 infection efficacy in BMSCs by immunofluorescence assay. (B) LV-BMP9 infection increased cell viability of BMSCs. (C) Expression of osteogenic factors, including BMP9, RUNX2, OCN, OPN, and OSX, was increased in LV-BMP9-infected BMSCs. # p<0.05 and ## p<0.01 versus the values of LV group. BMSCs – bone mesenchymal stem cells; RUNX2 – runt-related transcription factor 2; OCN – osteocalcin; OPN – osteopontin; OSX – osterix; BMP9 – bone morphogenetic protein.