Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

07 September 2020: Animal Study

A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors

Cheng Zhou 1BCDEF , Chuan Ye 2BCDF , Chen Zhao 1BCD , Junyi Liao 1BCF , Yuwan Li 1BC , Hong Chen 1BF , Wei Huang 1ADEG*

DOI: 10.12659/MSM.924666

Med Sci Monit 2020; 26:e924666

Figure 4 The LV-BMP9 infection enhanced cell viability and upregulated mRNA transcriptions of osteogenic factors (BMP9, RUNX2, OCN, OPN, OSX) in BMSCs. (A) Determination of LV or LV-BMP9 infection efficacy in BMSCs by immunofluorescence assay. (B) LV-BMP9 infection increased cell viability of BMSCs. (C) Expression of osteogenic factors, including BMP9, RUNX2, OCN, OPN, and OSX, was increased in LV-BMP9-infected BMSCs. # p<0.05 and ## p<0.01 versus the values of LV group. BMSCs – bone mesenchymal stem cells; RUNX2 – runt-related transcription factor 2; OCN – osteocalcin; OPN – osteopontin; OSX – osterix; BMP9 – bone morphogenetic protein.

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750