07 September 2020>: Animal Study
A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors
Cheng Zhou 1BCDEF , Chuan Ye 2BCDF , Chen Zhao 1BCD , Junyi Liao 1BCF , Yuwan Li 1BC , Hong Chen 1BF , Wei Huang 1ADEG*DOI: 10.12659/MSM.924666
Med Sci Monit 2020; 26:e924666
Figure 7 BMSCs-LV-BMP9-P3HB4HB composite bone repair material increased the expression of osteogenic factors, including BMP9, RUNX2, OCN, OPN, and OSX. (A) Expression of osteogenic factors evaluated by Western blot assay. (B) Effects of MSCs-LV-BMP9-P3HB4HB on RUNX2 expression. (C) Effects of MSCs-LV-BMP9-P3HB4HB on OCN expression. (D) Effects of MSCs-LV-BMP9-P3HB4HB on OPN expression. (E) Effects of MSCs-LV-BMP9-P3HB4HB on OSX expression. # p<0.05 versus the values of BMSC+LV-P3HB4HB group. BMSCs – bone mesenchymal stem cells; RUNX2 – runt-related transcription factor 2; OCN – osteocalcin; OPN – osteopontin; OSX – osterix; BMP9 – bone morphogenetic protein.