03 September 2020: Clinical Research
Positive Cross-Talk Between CXC Chemokine Receptor 4 (CXCR4) and Epidermal Growth Factor Receptor (EGFR) Promotes Gastric Cancer Metastasis via the Nuclear Factor kappa B (NF-κB)-Dependent Pathway
Yu Cheng 123ABCDEG , Xiaofang Che 123A , Simeng Zhang 123C , Tianshu Guo 123F , Xin He 123F , Yunpeng Liu 123AG* , Xiujuan Qu 123AG*DOI: 10.12659/MSM.925019
Med Sci Monit 2020; 26:e925019
Figure 1 CXCL12 and EGF cooperatively promote gastric cancer cell migration. (A) Protein levels of EGFR and CXCR4 in gastric cancer cells. (B, C) Relative mRNA and protein levels of CXCR4 and EGFR were evaluated after treatment with CXCL12 (100 ng/mL). (D, E) Relative mRNA and protein levels of CXCR4 and EGFR were evaluated after treatment with EGF (100 ng/mL). (F) MGC-803 and SGC-7901 cells were treated with CXCL12 (100 ng/mL) or EGF (100 ng/mL) or CXCL12 (100 ng/mL) plus EGF (100 ng/mL), cells migration was performed using the Transwell assay. Values are represented as mean±SD in 3 independent experiments. (* P<0.05, ** P<0.01).