03 September 2020: Clinical Research
Positive Cross-Talk Between CXC Chemokine Receptor 4 (CXCR4) and Epidermal Growth Factor Receptor (EGFR) Promotes Gastric Cancer Metastasis via the Nuclear Factor kappa B (NF-κB)-Dependent Pathway
Yu Cheng 123ABCDEG , Xiaofang Che 123A , Simeng Zhang 123C , Tianshu Guo 123F , Xin He 123F , Yunpeng Liu 123AG* , Xiujuan Qu 123AG*DOI: 10.12659/MSM.925019
Med Sci Monit 2020; 26:e925019
Figure 3 NF-κB transcription factor contributes to EGF/EGFR-mediated CXCR4 upregulation. (A) MGC-803 cells were treated with EGF (100 ng/mL). Phosphor-p65/IKKα/β were determined by western blot assay (m, minute; h, hour). (B) MGC-803 cells were incubated with EGF (100 ng/mL) for 1 h. Nuclear and cytoplasm cell lysate proteins were analyzed by western blot assay. (C) MGC-803 cells were treated with EGF (100 ng/mL) for 48 h and pretreated with or without BAY117082 (15 μM). Western blot analysis of CXCR4. (D) MGC-803 cells were treated with EGF (100 ng/mL) with or without BAY117082 (15 μM). Cell migration was examined by Transwell assay. Values are represented as mean±standard deviation (SD) in 3 independent experiments (* P<0.05). (E) MGC-803 cells were treated with EGF (100 ng/mL) for 48 h and pretreated with or without C225 (10 μg/mL). Western blot analysis of phosphor-p65/IKKα/β and CXCR4.