Figure 1 ROS was significantly increased in hypoxia-induced injury in rat primary neuron cells. (A) ROS level was detected by flow cytometry after 0, 6, 12, and 24 hours of hypoxic culture. (B) The mRNA expression of DUOX1 was detected by Q-PCR after 0, 6, 12, and 24 hours of hypoxic culture. (C) The protein expression of HIF-1α and DUOX1 was detected by western blot after 0, 6, 12, and 24 hours of hypoxic culture. * P<0.05, ** P<0.01, *** P<0.001 versus 0 hours 5% O2. ROS – reactive oxygen species; Q-PCR – quantitative polymerase chain reaction; DUOX1 – dual oxidase 1; HIF-1α – hypoxia-inducible factor-1α.