Figure 2 Vitamin D attenuated hypoxia-induced injury in rat primary neuron cells. (A) ROS level was detected by flow cytometry after treatment with different concentrations of vitamin D. (B) Apoptosis was detected by flow cytometry after treatment with different concentrations of vitamin D. (C, D) The mRNA expression of VDR and DUOX1 was detected by Q-PCR after treatment with different concentrations of vitamin D. (E) The protein expression of VDR and DUOX1 was detected by western blot after treatment with different concentrations of vitamin D. *** P<0.001 versus control; # P<0.05, ## P<0.01, ### P<0.001 versus 0 nM vitamin D. Control: rat neuron cells cultured under normoxic conditions. ROS – reactive oxygen species; VDR – vitamin D receptor; DUOX1 – dual oxidase 1; Q-PCR – quantitative polymerase chain reaction;