Figure 3 Downregulation of DUOX1 significantly attenuated hypoxia-induced injury in rat primary neuron cells. (A, B) The interference efficiency of DUOX1 was detected by Q-PCR (A) and western blot (B). (C, D) MDA (C) and SOD (D) levels were detected by biochemical detection. (E, F) ROS level (E) and apoptosis (F) were detected by flow cytometry. (G) Protein expression of NF-κB and cleaved caspase-3 was determined by western blot. * P<0.05, ** P<0.01, *** P<0.001 versus siNC or control; # P<0.05, ## P<0.01, ### P<0.001 versus 5% O2+siNC. siNC: Negative control of DUOX1 interference; siDUOX1: DUOX1 interference; Control: rat neuron cells cultured under normoxic conditions. DUOX1 – dual oxidase 1; Q-PCR – quantitative polymerase chain reaction; MDA – malondialdehyde; SOD – superoxide dismutase; ROS – reactive oxygen species.