Figure 2 HG-trigged podocyte apoptosis, fibrosis and inflammation were relieved by the elevation of 4930556M19Rik. Podocytes were treated with HG (30 mM glucose), NC (5-mM glucose+24.5-mM mannitol) or transfected with pcDNA or 4930556M19Rik in HG. (A) 4930556M19Rik expression in podocytes was determined by qRT-PCR assay. (B) Apoptosis of podocytes was tested by flow cytometry analysis. (C) Protein levels of α-SMA, FN, Col I, and podocin were measured with Western blot assay. (D–F) Levels of IL-1β, TNF-α, and IL-6 were examined using ELISA kits. * P<0.05.