Figure 4 4930556M19Rik overexpression suppressed cell apoptosis, fibrosis, and inflammatory response by binding to miR-27a-3p in HG-stimulated podocytes. Podocytes were transfected with pcDNA, 4930556M19Rik, 4930556M19Rik+miR-NC or 4930556M19Rik+miR-27a-3p followed by treatment of HG. (A) MiR-27a-3p expression in HG-induced podocytes was measured by qRT-PCR assay. (B) Apoptosis in HG-induced podocytes was tested by via flow cytometry analysis. (C) Protein levels of α-SMA, FN, Col I, and podocin in HG-induced podocytes were measured using Western blot assay. (D–F) Secretion of IL-1β, TNF-α, and IL-6 was examined with ELISA kits. * P<0.05.