Figure 6 TIMP3 knockdown reversed the suppressive roles of miR-27a-3p inhibition in cell apoptosis, fibrosis, and inflammation in HG-stimulated podocytes. Podocytes were introduced with in-miR-NC, in-miR-27a-3p, in-miR-27a-3p+si-NC or in-miR-27a-3p+si-TIMP3 followed by HG treatment. (A) The protein level of TIMP3 in HG-induced podocytes was measured by Western blot assay. (B) Cell apoptosis in HG-induced podocytes was assessed by flow cytometry analysis. (C) The protein levels of α-SMA, FN, Col I, and podocin in HG-induced podocytes were detected via Western blot assay. (D–F) Levels of IL-1β, TNF-α, and IL-6 were determined with ELISA kits. * P<0.05.