21 September 2020 : Animal Research
P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury
Xiao Fan12ABCEF, Wei Ma3ABCEF, Yingyu Zhang1BCD, Li Zhang24AFG*DOI: 10.12659/MSM.925491
Med Sci Monit 2020; 26:e925491
Figure 2 Western blot and qPCR assay were used to assess the expression of P2X7R protein and P2X7R mRNA in spinal cord. (A) Western blot assay showed that the expression of P2X7R protein in spinal cord was increased notably after spinal cord injury compared to the sham group (** P<0.01) and, compared to model group, BzATP increased the expression of P2X7R protein in spinal cord (* P<0.05) while A-438079 significantly decreased the expression of P2X7R protein in spinal cord (** P<0.01). (B) qPCR assay showed that the expression of P2X7R mRNA in spinal cord increased notably after spinal cord injury compared to the sham group (** P<0.01), and compared to the model group, BzATP increased the expression of P2X7R mRNA in spinal cord (* P<0.05) while A-438079 significantly decreased the expression of P2X7R mRNA in spinal cord (** P<0.01).