Figure 7 (A) Regulatory splicing factor (SF)-alternative splicing (AS) network based on strong correlations between percent spliced in (PSI) value of splicing events and mRNA expression of SFs (|cor| >0.6). Favorable (purple circles) or unfavorable (orange circles) AS events were positively (red lines) or negatively (green lines) regulated by SFs (yellow triangles). (B–E) Kaplan-Meier survival analysis validated the prognostic value of SFs. Four SFs (ELAVL4, ESRP1, RBM5, and TRA2A) were significantly associated with overall survival of pancreatic ductal carcinoma patients (P<0.05). (F–M) Representative dot plots showing the correlation between expression of prognostic SFs (ELAVL4, ESRP1, RBM5, and TRA2A) and PSI values of splicing events.