Figure 4 Histomorphological and apoptotic changes of cornea, iris, ciliary body, and anterior chamber induced by chronic ocular hypertension (COH). (A–D) The frozen sections and TUNEL staining of the anterior segment (A–C represent cornea, iris, and ciliary body, respectively; magnification 200, scale bar=100 μm; (D) Anterior chamber angle, magnification 100, scale bar=100 μm). (E) There was no significant difference in the number of TUNEL-positive corneal endothelial cells (CECs) in the corneas at 2 and 4 weeks after ocular hypertension compared with that of the control group (n=6, P=0.503, 0.207, respectively). At 8 weeks after ocular hypertension, the number of CECs positive for TUNEL staining increased in a single field of view compared with the control group.