07 September 2020>: Lab/In Vitro Research
Reduced Caudal Type Homeobox 2 (CDX2) Promoter Methylation Is Associated with Curcumin’s Suppressive Effects on Epithelial-Mesenchymal Transition in Colorectal Cancer Cells
Ting Chen 1ABCDEF , Chun Yang 2ABCDEFG* , Zhenli Xi 3ABDEF , Fen Chen 1ABCDE , Hailin Li 2ABCDEDOI: 10.12659/MSM.926443
Med Sci Monit 2020; 26:e926443
Figure 4 (A) Columns indicate the relative mRNA expression levels of cdx2 and wnt3a in SW480 cells incubated with curcumin at 0, 0.1, 0.2, or 0.4 μmol/L for 24 h. (B) Immunoblots of CDX2, Wnt3a, and GAPDH. Columns indicate the relative expression levels of CDX2 and Wnt3a in SW480 cells incubated with curcumin at 0, 0.1, 0.2, or 0.4 μmol/L for 24 h. (C) Immunoblots of β-catenin and histone H3. Columns indicate the relative nuclear translocation levels of β-catenin (β-catenin/histone H3) in SW480 cells incubated with curcumin at 0, 0.1, 0.2, or 0.4 μmol/L for 24 h. (D) Captured images of immunofluorescence staining of β-catenin (stained by Alexa-488, green fluorescence), cell nuclei (stained by DAPI, blue fluorescence) and their merge were demonstrated. Columns indicate the relative nuclear translocation levels (translocated cell count/total cell count) in SW480 cells incubated with curcumin at 0, 0.1, 0.2, or 0.4 μmol/L for 24 h. (n=6, * P<0.05).