Figure 7 Omipalisib decreased phosphorylation of proteins in mTOR and ERK signaling pathway. (A) Esophageal squamous cell carcinoma (ESCC) cells were treated with the various concentrations of omipalisib for 24 hours. The protein expression levels of 4EBP1, p-4EBP1, p70S6K, p-p70S6k, S6, p-S6, AKT, and p-AKT were identified by Western blot analysis. (B) ERK and p-ERK protein levels in the 4 ESCC cell lines treated with omipalisib at the indicated concentrations for 24 hours. (C) Density analysis of protein bands performed with ImageJ software. Plot is the average of 3 independent experiments. (* P<0.05, ** P<0.01, *** P<0.001).