Figure 1 NEAT1 is upregulated in the placenta of PE rats. (A) Immunofluorescence staining, showing the percentages of cells positive for cytokeratin 7 and vimentin proteins; (B) RT-qPCR, showing the expression of NEAT1 mRNA in placental tissues (** p<0.01 by unpaired t test); (C) RT-qPCR, showing the transfection efficiency of pcDNA-NEAT1 and siRNA-NEAT1 (** p<0.01 by 1-way ANOVA); (D) cell proliferation ability, measured by CCK-8 (* p<0.05 by 2-way ANOVA) and colony formation (* p<0.05 by 1-way ANOVA) assays; (E) cell invasion and migration activities measured by Transwell assays (* p<0.05 by 1-way ANOVA); (F) caspase-3 and caspase-9 activities (* p<0.05 by 1-way ANOVA); (G) cell apoptosis, measured by flow cytometry (* p<0.05 by 1-way ANOVA). Each figure represents the average of 3 replicates.