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23 October 2020: Animal Study

Long Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 (NEAT1) Represses Proliferation of Trophoblast Cells in Rats with Preeclampsia via the MicroRNA-373/FLT1 Axis

Lingling Teng 1ABEF* , Pingping Liu 2ABDE* , Xiao Song 3BCE , Hui Wang 4CEF , Jing Sun 5BDE , Zhongxia Yin 6CDEF*

DOI: 10.12659/MSM.927305

Med Sci Monit 2020; 26:e927305

Figure 1 NEAT1 is upregulated in the placenta of PE rats. (A) Immunofluorescence staining, showing the percentages of cells positive for cytokeratin 7 and vimentin proteins; (B) RT-qPCR, showing the expression of NEAT1 mRNA in placental tissues (** p<0.01 by unpaired t test); (C) RT-qPCR, showing the transfection efficiency of pcDNA-NEAT1 and siRNA-NEAT1 (** p<0.01 by 1-way ANOVA); (D) cell proliferation ability, measured by CCK-8 (* p<0.05 by 2-way ANOVA) and colony formation (* p<0.05 by 1-way ANOVA) assays; (E) cell invasion and migration activities measured by Transwell assays (* p<0.05 by 1-way ANOVA); (F) caspase-3 and caspase-9 activities (* p<0.05 by 1-way ANOVA); (G) cell apoptosis, measured by flow cytometry (* p<0.05 by 1-way ANOVA). Each figure represents the average of 3 replicates.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750