23 October 2020 : Animal Research
Long Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 (NEAT1) Represses Proliferation of Trophoblast Cells in Rats with Preeclampsia via the MicroRNA-373/FLT1 Axis
Lingling Teng1ABEF, Pingping Liu2ABDE, Xiao Song3BCE, Hui Wang4CEF, Jing Sun5BDE, Zhongxia Yin6CDEF*DOI: 10.12659/MSM.927305
Med Sci Monit 2020; 26:e927305
Figure 5 NEAT1 modulates FLT1 via miR-373. (A) RT-qPCR measurements of FLT1 mRNA expression in cells transfected with siRNA-NEAT1 alone or in the presence of miR-373 inhibitor (* p<0.05 by 1-way ANOVA); (B) RT-qPCR measurements of FLT1 mRNA expression in cells transfected with pcDNA-NEAT1 with or without siRNA-FLT1 (* p<0.05 by 1-way ANOVA); (C) cell proliferation activity, measured by the CCK-8 method (* p<0.05 by 2-way ANOVA) and colony formation assay (* p<0.05 by 1-way ANOVA); (D) cell invasion and migration activities, measured by Transwell assays (* p<0.05 by 1-way ANOVA); (E) cell apoptosis, measured by flow cytometry (* p<0.05 by 1-way ANOVA). Each figure represents the average of 3 replicates.