28 October 2020: Lab/In Vitro Research
Upregulation of Serine Proteinase Inhibitor Clade B Member 3 (SERPINB3) Expression by Stromal Cell-Derived Factor (SDF-1)/CXCR4/Nuclear Factor kappa B (NF-κB) Promotes Migration and Invasion of Gastric Cancer Cells
Jing Gong 1ABCDEF* , Yongxi Song 2AB* , Ling Xu 3ACEF , Xiaofang Che 3DEF , Kezuo Hou 3BC , Tianshu Guo 3BC , Yu Cheng 3ABG , Yunpeng Liu 3ADE* , Xiujuan Qu 3AG*DOI: 10.12659/MSM.927411
Med Sci Monit 2020; 26:e927411
Figure 2 SDF-1 upregulated SERPINB3 protein expression in gastric cancer cells. (A) Expression microarray results showed that mRNA levels of SERPINB3 were elevated in MGC803 cells. (B) CXCR4 and SERPINB3 were expressed in a variety of gastric cancer cell lines. (C) MGC803 and BGC823 cells were treated with SDF-1 (100 ng/mL), and SERPINB3 protein expression increased, as detected by western blot analysis. (D) RT-PCR showed that the mRNA level also increased after treatment with SDF-1. (E) Increased mRNA level was also verified in the TCGA database. (F) KMplot database (http://kmplot.com/analysis/) 209 719 dataset results suggested that elevated SERPINB3 expression (probe expression greater than 107) in patients with gastric cancer indicated short overall survival and poor prognosis (P=0.0018).
- Download PDF
- Order reprints
- Export Article
- Related articles
- Share by email
- Metrics