28 October 2020>: Lab/In Vitro Research
Upregulation of Serine Proteinase Inhibitor Clade B Member 3 (SERPINB3) Expression by Stromal Cell-Derived Factor (SDF-1)/CXCR4/Nuclear Factor kappa B (NF-κB) Promotes Migration and Invasion of Gastric Cancer Cells
Jing Gong 1ABCDEF* , Yongxi Song 2AB* , Ling Xu 3ACEF , Xiaofang Che 3DEF , Kezuo Hou 3BC , Tianshu Guo 3BC , Yu Cheng 3ABG , Yunpeng Liu 3ADE* , Xiujuan Qu 3AG*DOI: 10.12659/MSM.927411
Med Sci Monit 2020; 26:e927411
Figure 4 NF-κB was involved in SDF-1-induced elevated SERPINB3 expression during gastric cancer cell migration and invasion. (A) Serum-starved MGC803 and BGC823 cells were treated with SDF-1 (100 ng/mL) for 15 min, 30 min, 1 h, or 2 h, and the total protein levels of P65, p-P65, IκB, and p-IκB were detected using western blot analysis. (B, C) P65 was transported to the nuclei of MGC803 cells after SDF-1 (100 ng/ml) treatment by the method of nuclear pulp separation and immunofluorescence. (D) With or without 6-hours Bay117082 (15 μM) pretreatment, the activation of the NF-κB pathway (E) and the increased SERPINB3 level were evaluated by western blot analysis. (F) Migration and invasion abilities were assessed using a Transwell assay with or without the inhibition of NF-κB. Data are presented as the mean±standard deviation of the results from 3 independent experiments. * P<0.05.
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