Figure 5 CXCR4 was involved in SDF-1-induced activation of NF-κB and elevated SERPINB3 levels, which resulted in the migration and invasion of gastric cancer cells. (A) Serum-starved MGC803 and BGC823 cells were treated with SDF-1 (100 ng/mL) with or without pretreatment with AMD3100 (10 μg/mL) and activation of the NF-κB pathway. (B) Nuclear translocation of P65 was also inhibited by AMD3100 in MGC803 cells. (C) With or without 2 hours of AMD3100 pretreatment, the increased SERPINB3 level was evaluated by western blot analysis. (D) Migration and invasion abilities were assessed using a Transwell assay with or without the inhibition of AMD3100. Data are presented as the mean±standard deviation of the results from 3 independent experiments. * P<0.05.