Figure 1 Study flow chart. On the basis of the DAVID database, we predicted the proteins and pathways associated with asthma and erectile dysfunction (ED). We used real-time quantitative polymerase chain reaction and western blotting to measure expression of insulin, albumin, vascular endothelial growth factor A, tumor necrosis factor, and interleukin 6 proteins and messenger ribonucleic acid in rat penis cells of each group. The results of in vivo experiments were consistent with the prediction results of bioinformatics analysis. Asthma can induce systemic inflammation and extensive vascular endothelial damage, which then leads to ED.