02 January 2021>: Clinical Research
Regulatory Effect of PD1/PD-Ligand 1 (PD-L1) on Treg Cells in Patients with Idiopathic Pulmonary Fibrosis
Bing Wang 1ABCE , Wenmei Bai 2BEF , Hongxia Ma 2BDE , Fengsen Li 1ADG*DOI: 10.12659/MSM.927577
Med Sci Monit 2021; 27:e927577
Figure 2 The effect of cell fibrosis on Treg cells differentiation. (A) Changes of Treg cells proportion in IPF patients was the most obvious. (B) Detection of cytokines in blood of IPF patients. Among them, the changes of IL-4 and IL-10 were the most significant. (C) TGF-beta stimulates pulmonary fibroblasts to obtain myofibroblasts. (TGF-beta concentration: 1 ug/ml) (100×). (D) The purity of CD4+ T cells isolated from peripheral blood by magnetic beads was more than 90%. (E) The proportion of Treg cells decreased significantly after CD4+ T cells co-culture with myofibroblasts for 24 h. ** P<0.01, *** P<0.001.