26 March 2021>: Animal Study
Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) PathwayYanhua Guan 12ABCDEF , Xu Wang 13ABCDEF*
Med Sci Monit 2021; 27:e928153
Figure 3 Effect of salvianic acid A (SAA) at different concentrations on the expression level of Akt/eNOS pathway was detected by western blotting. (A, B) Western blotting was performed to detect the protein levels of Akt, p-Akt, eNOS, and p-eNOS. *** P<0.001 compared to the control group. ## P<0.01 compared to the high-glucose (HG) group. (C) The ratio of p-Akt/Akt. *** P<0.001 compared to the control group. # P<0.05 compared to the HG group. (D) The ratio of p-eNOS/eNOS. *** P<0.001 compared to the control group. # P<0.05 compared to the HG group. The cells were divided into 4 groups: control group (5.5 mmol/L glucose and 25 mmol/L mannitol), HG group (30 mmol/L glucose), HG+50 group (cells were pretreated with 50 μmol/L SAA for 24 h and then cultured with 30 mmol/L glucose for 24 h) and HG+100 group (cells were pretreated with 100 μmol/L SAA for 24 h and then cultured with 30 mmol/L glucose for 24 h).