14 January 2021 : Laboratory Research
Mechanical Stress Affects Circadian Rhythm in Skeletal Muscle (C2C12 Myoblasts) by Reducing Per/Cry Gene Expression and Increasing Bmal1 Gene Expression
Mengjia Wang1BCDEF, Da Yu2BCDF, Lichun Zheng1BCDF, Bing Hong1BCD, Houxuan Li1BCD, Xiaobei Hu1BC, Kun Zhang1BF, Yongbin Mou1ADEG*DOI: 10.12659/MSM.928359
Med Sci Monit 2021; 27:e928359
Figure 3 Determination of the Per and Cry gene transcriptions and protein expressions in C2C12 myoblasts (n=6). (A) Statistical analysis for Clock gene transcription identified by PCR assay. (B) Statistical analysis for Bmal1 gene transcription of Cry identified by PCR assay. (C) Western blot images for Clock and Bmal1 protein expression. (D) Statistical analysis for Clock expression in C2C12 cells. (E) Statistical analysis for Bmal1 expression in C2C12 cells. * P<0.05 and ** P<0.001 represent differences illustrated in images.