09 March 2021 : Laboratory Research
Gasdermin D Protects Mouse Podocytes Against High-Glucose-Induced Inflammation and Apoptosis via the C-Jun N-Terminal Kinase (JNK) Pathway
Huifang Li1ABCDE*, Kunxiao Zhao2ADF, Ying Li1ADFGDOI: 10.12659/MSM.928411
Med Sci Monit 2021; 27:e928411
Figure 2 Effects of GSDMD gene knockout on inflammation and apoptosis in mouse podocytes induced by HG. (A–D) The expression of siGSDMD protein was detected by western blot. (E–G) The activity of Bax, Bcl-2, cleaved caspase-3, IL-1β, IL-6, and TNF-α was evaluated by western blotting. (H–J) The activities of synaptopodin in mouse podocytes was evaluated by western blotting and immunofluorescence. NC – siRNA negative control; siRNA – GSDMD siRNA (20 μM); NG – normal-glucose; HG – high-glucose; HG+NC – high-glucose+siRNA negative control; HG+siGSDMD – high-glucose (30mM)+GSDMD siRNA (20 μM). Date represent the average of 3 measurements. Mean±SD of 3 experiments is shown. * P<0.05, ** P<0.01 vs NG, # P<0.05 vs HG.