Figure 4 Effect of GSDMD knockdown on HG-induced mitochondrial ROS production and p-JNK expression levels in mouse podocytes. (A) Mitochondrial ROS production was measured using a confocal microscope. (B, C) SOD2 activity was evaluated by western blotting. (D, E) The protein activity of JNK and p-JNK was detected by western blotting. NG – normal-glucose; HG – high-glucose; HG+siGSDMD – high-glucose+siGSDMD; HG+SP – high-glucose+SP600125. Data represent the average of 3 measurements. Mean±SD of 3 experiments is shown. (C) * P<0.05 vs NG, # P<0.05 vs HG; (E) * P<0.05 vs HG, # P<0.05 vs HG.