12 March 2021: Lab/In Vitro Research
Citrus Alkaline Extracts Inhibit Senescence of A549 Cells to Alleviate Pulmonary Fibrosis via the β-Catenin/P53 Pathway
Di Han 1BDE , Yong Xu 1CE , Wen-Pan Peng 1BD , Fanchao Feng 12AD , Zhichao Wang 1BD , Cheng Gu 1BD , Xianmei Zhou 12AG*DOI: 10.12659/MSM.928547
Med Sci Monit 2021; 27:e928547
Figure 4 Citrus alkaline extracts (CAE) inhibited A549 senescence through the β-catenin/p53 pathway. (A) The analysis of p53and p21 in A549 cells by western blot. Each group was assessed in triplicate, and experiments were repeated 3 times. Bar graphs show the relative quantification, ### P<0.001, vs Blank, **P<0.01, ***P<0.001, and ns = not significant vs Adriamycin RD (ARD); s, P<0.05, N=not significant vs ARD+CAE. (B) Real-time qPCR analysis of genes; relative expression of p53 and p21. #### P<0.0001 vs Blank, **** P<0.0001 vs ARD; s, P<0.05. N=not significant vs ARD+CAE. (C) Western blot analysis of β-catenin and GSK-3β in A549 cells. Each group was assessed in triplicate and experiments were repeated 3 times. Bar graphs indicate the relative quantification. #### P<0.0001 vs Blank, **** P<0.0001, ns=not significant vs ARD; s, P<0.05. N=no significant vs ARD+CAE. (D) The qPCR analysis of real-time genes relative, expression of p16. ## P<0.01 vs Blank, ** P<0.05, **** P<0.0001, and ns=not significant vs ARD; s, P<0.05. N=no significant vs ARD+CAE.