Figure 5 Citrus alkaline extracts (CAE) reduced expression levels of fibrosis markers in MRC-5 cells by inhibiting senescence in A549 cells. (A) An analysis of α-SMA by western blot, collagen I, collagen III in MRC-5 cells. Each group was assessed in triplicate, and experiments were repeated 3 times. Bar graphs show the relative quantification, # P<0.05 vs Blank, ** P<0.01, and ns=not significant vs Adriamycin RD (ARD). (B) Real-time qPCR analysis of genes, and relative expression of α-SMA, collagen I, and collagen III. ### P<0.001, #### P<0.0001 vs Blank, * P<0.05, ** P<0.01, **** P<0.0001, and ns=no significant vs ARD. (C) Determination of transforming collagen I and collagen III in the cell culture supernatant. The data are shown as mean±SD. #### P<0.0001 vs Blank; ns=no significant ** P<0.01 and **** P<0.0001 vs ARD.