27 February 2021 : Laboratory Research
Collagen Type X Alpha 1 (COL10A1) Contributes to Cell Proliferation, Migration, and Invasion by Targeting Prolyl 4-Hydroxylase Beta Polypeptide (P4HB) in Breast Cancer
Weibin Yang1ABCDEF*, Xuan Wu1ABCEF, Fan Zhou1ABCEDOI: 10.12659/MSM.928919
Med Sci Monit 2021; 27:e928919
Figure 2 Upregulation of COL10A1 enhanced cell proliferation and clone-forming abilities of breast cancer cells. (A) Western blot analysis was performed to detect the transfection efficiency in MCF-7 cells which were transfected with pcDNA-COL10A1. (B) RT-qPCR analysis was performed to detect the transfection efficiency in MCF-7 cells which were transfected with pcDNA-COL10A1. (C) CCK-8 assay was applied to explore the effect of COL10A1 overexpression on cell viability of MCF-7. (D) Colony formation assay was applied to explore the effect of COL10A1 overexpression on clone-forming ability of MCF-7. (E) Western blot analysis was performed to detect the transfection efficiency in MDA-MB-231 cells which were transfected with pcDNA-COL10A1. (F) RT-qPCR analysis was performed to detect the transfection efficiency in MDA-MB-231 cells which were transfected with pcDNA-COL10A1. (G) CCK-8 assay was applied to explore the effect of COL10A1 overexpression on cell viability of MDA-MB-231. (H) Colony formation assay was applied to explore the effect of COL10A1 overexpression on clone-forming ability of MDA-MB-231. * P<0.05, ** P<0.01, *** P<0.001.