27 February 2021>: Lab/In Vitro Research
Collagen Type X Alpha 1 (COL10A1) Contributes to Cell Proliferation, Migration, and Invasion by Targeting Prolyl 4-Hydroxylase Beta Polypeptide (P4HB) in Breast Cancer
Weibin Yang ABCDEF* , Xuan Wu ABCEF , Fan Zhou ABCEDOI: 10.12659/MSM.928919
Med Sci Monit 2021; 27:e928919
Figure 6 COL10A1 combined with P4HB. (A) P4HB expression in tumor tissues or normal tissues of breast cancer patients was analyzed based on GEPIA repository (http://gepia.cancer-pku.cn/). (B) Western blot analysis of P4HB expression in human breast epithelial cells (MCF-10A) and breast cancer cell lines (MCF-7, MDA-MB-231, SUM190PT, SK-BR-3). (C) RT-qPCR analysis of P4HB expression in human breast epithelial cells (MCF-10A) and breast cancer cell lines (MCF-7, MDA-MB-231, SUM190PT, SK-BR-3). (D, E) The relation between COL10A1 and P4HB was determined by Co-IP assay. (F) Western blot analysis of P4HB expression after transfection with pcDNA-COL10A1. * P<0.05, *** P<0.001.