01 June 2021 : Animal Research
Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating Nuclear Factor Erythroid 2-Related Factor 2 (NRF2)
HuaiMing Wang1A, YiQuan Xu1B, Shuying Zhu1E, XueMing Li2F, HongWei Zhang1G*DOI: 10.12659/MSM.930042
Med Sci Monit 2021; 27:e930042
Figure 4 Sevoflurane post-treatment reduces the levels of G9a and H3K9me2 in OGD-induced PC12 cells to alleviate OGD-induced cell damage. (A) The protein levels of G9a and H3K9me2 in cells of each group were detected by western blot analysis. (B) ELISA was utilized to measure the levels of inflammatory factors (TNF-α, IL-1β, IL-6, and IL-8) in cells of each group. (C) Flow cytometry was performed to detect the apoptosis rate of cells in each group. The data are expressed as the mean±standard deviation. The data among groups were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons test. * P<0.05.