14 June 2021 : Laboratory Research
Helenalin Facilitates Reactive Oxygen Species-Mediated Apoptosis and Cell Cycle Arrest by Targeting Thioredoxin Reductase-1 in Human Prostate Cancer Cells
Mei Yang1A, Weihua Zhang2B, Xiuxiu Yu3C, Feng Wang3D, Yeping Li3E, Yan Zhang3F, Yu Yang3AG*DOI: 10.12659/MSM.930083
Med Sci Monit 2021; 27:e930083
Figure 3 Helenalin induces ROS-mediated apoptosis as well as cell cycle arrest in prostate cancer cells. (A–D) Apoptotic levels were determined in DU145 and PC-3 cells treated with helenalin and/or ROS inhibitor. **** P<0.0001. (E–H) Cell cycle was assessed in DU145 and PC-3 cells following treatment with helenalin and/or ROS inhibitor. (I–M) Western blot was carried out for examination of Bcl-2 and Bax expressions in DU145 and PC-3 cells following treatment with helenalin and/or ROS inhibitor. Compared to controls, *P<0.05; *** P<0.001, and **** P<0.0001. Compared to helenalin group, ns: not significant; ### P<0.001; #### P<0.0001.