14 June 2021 : Laboratory Research
Helenalin Facilitates Reactive Oxygen Species-Mediated Apoptosis and Cell Cycle Arrest by Targeting Thioredoxin Reductase-1 in Human Prostate Cancer Cells
Mei Yang1A, Weihua Zhang2B, Xiuxiu Yu3C, Feng Wang3D, Yeping Li3E, Yan Zhang3F, Yu Yang3AG*DOI: 10.12659/MSM.930083
Med Sci Monit 2021; 27:e930083
Figure 4 Helenalin treatment significantly suppresses TrxR1 expression in prostate carcinoma cells. (A, B) RT-qPCR was utilized to validate the knockdown effects of siRNAs against TrxR1 in 2 prostate carcinoma cell lines. (C, D) The overexpression effects of TrxR1 plasmid were verified in 2 prostate carcinoma cell lines by RT-qPCR. (E, F) RT-qPCR was performed to determine the mRNA expression of TrxR1 in 2 prostate carcinoma cells exposed to si-TrxR1, TrxR1 plasmid, and/or helenalin. (G–I) The protein expression of TrxR1 was assessed in the 2 cell lines treated with si-TrxR1, TrxR1 plasmid, and/or helenalin via western blot. ns: not significant; * P<0.05; ** P<0.01, and **** P<0.0001.