14 June 2021 : Laboratory Research
Helenalin Facilitates Reactive Oxygen Species-Mediated Apoptosis and Cell Cycle Arrest by Targeting Thioredoxin Reductase-1 in Human Prostate Cancer Cells
Mei Yang1A, Weihua Zhang2B, Xiuxiu Yu3C, Feng Wang3D, Yeping Li3E, Yan Zhang3F, Yu Yang3AG*DOI: 10.12659/MSM.930083
Med Sci Monit 2021; 27:e930083
Figure 6 Helenalin treatment induces apoptosis and cell cycle arrest through suppressing TrxR1 expression for prostate carcinoma cells. (A–D) Apoptotic levels were examined in 2 prostate carcinoma cells exposed to si-TrxR1, TrxR1 plasmid and/or helenalin by flow cytometry. **** P<0.0001. (E–H) Cell cycle was evaluated in the 2 prostate cancer cells following treatment with si-TrxR1, TrxR1 plasmid, and/or helenalin by flow cytometry. Compared to si-NC, **** P<0.0001. Compared to si-TrxR1, # P<0.05 and #### P<0.0001. Compared to empty vector, &&&& P<0.0001. Compared to TrxR1, @@@@ P<0.0001.