11 June 2021 : Animal Research
Protective Effects of Bone Marrow Mesenchymal Stem Cells (BMMSCS) Combined with Normothermic Machine Perfusion on Liver Grafts Donated After Circulatory Death via Reducing the Ferroptosis of Hepatocytes
Dong Sun1BCDEF, Liu Yang1BCDEF, Weiping Zheng23CE, Huan Cao14BD, Longlong Wu5DF, Hongli Song26AG*DOI: 10.12659/MSM.930258
Med Sci Monit 2021; 27:e930258
Figure 2 Evaluation of the NMP system and BMMSCs. (A) Morphology characteristics of third-generation BMMSCs; (B) Adipogenic induction; (C) Osteogenic induction; (D) Bright-field image of GFP-BMMSCs; (E) Fluorescent image of GFP-BMMSCs showing that >85% of the cells are transduced with GFP; (F) Fluorescent image of DCD donor liver perfused by BMMSCs combined with NMP at 6 h, the arrow indicates GFP-BMMSCs colonized in hepatic sinusoids. BMMSCs – bone marrow mesenchymal stem cells; GFP – green fluorescent protein; NMP – normothermic machine perfusion.