Figure 1 BTVs exerted an antitumor effect against a range of renal tumor cell lines. Morphological changes in the renal cancer cell lines and human primary proximal tubular epithelial cell (HPTEC). (A) After 72 h of infection by BTVs (MOI 10 PFUs/cell), cytopathic effects (CPE) were clearly observed in all cancer cell lines, but not in HPTECs (×200). (B) Cells were infected with BTVs at MOIs between 0.001 and 10, and cell survival was measured by a CCK-8 assay at 24, 48, and 72 h after infection. For each cell line, the data were normalized to the uninfected control (no virus). Data are representative of at least 3 biological replicates. Columns, mean; bars, SEM.