28 January 2021 : Animal Research
Bluetongue Viruses Act as Novel Oncolytic Viruses to Effectively Inhibit Human Renal Cancer Cell Growth In Vitro and In Vivo
Haozhou Wang1ABCE, Liming Song1DEF, Xin Zhang1BCF, Xiaodong Zhang1AF, Xiaoguang Zhou1ABG*DOI: 10.12659/MSM.930634
Med Sci Monit 2021; 27:e930634
Figure 1 BTVs exerted an antitumor effect against a range of renal tumor cell lines. Morphological changes in the renal cancer cell lines and human primary proximal tubular epithelial cell (HPTEC). (A) After 72 h of infection by BTVs (MOI 10 PFUs/cell), cytopathic effects (CPE) were clearly observed in all cancer cell lines, but not in HPTECs (×200). (B) Cells were infected with BTVs at MOIs between 0.001 and 10, and cell survival was measured by a CCK-8 assay at 24, 48, and 72 h after infection. For each cell line, the data were normalized to the uninfected control (no virus). Data are representative of at least 3 biological replicates. Columns, mean; bars, SEM.