28 January 2021 : Animal Research
Bluetongue Viruses Act as Novel Oncolytic Viruses to Effectively Inhibit Human Renal Cancer Cell Growth In Vitro and In Vivo
Haozhou Wang1ABCE, Liming Song1DEF, Xin Zhang1BCF, Xiaodong Zhang1AF, Xiaoguang Zhou1ABG*DOI: 10.12659/MSM.930634
Med Sci Monit 2021; 27:e930634
Figure 4 BTV induces apoptosis in renal cancer cells. (A) Apoptosis was analyzed by flow cytometry. The HPTEC, CAKI-1, and OR-SC-2 cells were pre-treated with a pan-caspase inhibitor Z-VAD-FMK (10 μM) for 24 h before BTV infection (MOI=10, 24 h). The untreated cells served as control. (B). Quantification of apoptosis. The cell population in the right 2 quadrants (Annexin V-positive) correspond to the apoptotic cells. Columns, mean; bars, SEM. (C) The ladder-like pattern of DNA fragmentation was observed in BTV-infected CAKI-1 cells at 48, 72, and 96 h after BTV infection (MOI=10).