Figure 2 Detection of liver damage in mice by hematoxylin-eosin staining (H&E staining) at 24 h after intraperitoneal injection of CCl4. (A–F) Liver damage of the female normal group, the male normal group, the female normal control group, the male normal control group, the female CCl4 group, and the male CCl4 group, respectively. (G) Area of necrosis. Measurements of liver sections of at least 10 mm2 per mouse were performed using ImagePro Plus 6.0 software. ** P<0.01: there was a significant difference between the female group and the male group. ## P<0.01: there was a significant difference between the female CCl4 group and the normal group or the normal control group. && P<0.01: there was a significant difference between the male CCl4 group and the normal group or the normal control group. Experiments were repeated in triplicate (scale bar, 50 μm). H&E – hematoxylin and eosin.