Figure 3 Detection of glycogen expression by periodic acid Schiff reagent staining (PAS staining) assay of staining at 24 h after intraperitoneal injection of CCl4. (A–F) Liver PAS staining of the female normal group, the male normal group, the female normal control group, the male normal control group, the female CCl4 group, and the male CCl4 group, respectively. (G) Uniform white balance processing, two-color segmentation, and optical density analysis using Motic Images Advanced 3.2 software. At least 12 mm2 of liver tissue sections were tested for each mouse. ** P<0.05: there was a significant difference between the female group and the male group. ## P<0.01: there was a significant difference between the female CCl4 group and the normal group or the normal control group. && P<0.01: there was a significant difference between the male CCl4 group and the normal group or the normal control group. Experiments were repeated in triplicate (scale bar, 50 μm). PAS – periodic acid-Schiff.