Figure 4 Expression of HSP27, HSP70, PCNA, Bcl-2, Bax, caspase-3, and CYP2E1 in the liver of mice at 24 h after intraperitoneal injection of CCl4. Expression of HSP27, HSP70, PCNA, Bcl-2, Bax, pro-caspase-3, cleaved caspase-3 and CYP2E1 protein was detected by western blot analysis. The protein bands were quantified for (A) HSP27, (B) HSP70, (C) PCNA, (D) Bcl-2, (E) Bax, (F) caspase-3, and (G) CYP2E1 with Gel-Pro Analyzer 4.0 software (Media Cybernetics Inc.), and the intensities of the bands were normalized against beta-actin. Experiments were performed in triplicate, and all experimental data are expressed as the mean±standard deviation (SD). ** P<0.01 or * P<0.05: there was a significant difference between the female group and the male group. ## P<0.01 or # P<0.05: the female carbon tetrachloride group was significantly different from the normal group or the normal control group. && P<0.01 or & P<0.05: there was a significant difference between the male carbon tetrachloride group and the normal group or the normal control group. AU – arbitrary unit; NG – normal group; NCG – normal control group; CTG – intraperitoneal injection of CCl4 group.