16 December 2021>: Animal Study
Forkhead Box Protein M1 Promotes Nasopharyngeal Carcinoma Cell Tumorigenesis Possibly via the Wnt/β-Catenin Signaling Pathway
Chao Yu 1ABEFG* , Hongyan Chen 2AEF , Yanli Zhao 1B , Yuedong Zhang 1BCDDOI: 10.12659/MSM.931970
Med Sci Monit 2021; 27:e931970
Figure 2 Effect of inhibition of FoxM1 on NPC cell apoptosis and nuclear morphology(A) CNE-1 and CNE-2 cells were exposed to vehicle or 8 μM of thiostrepton for 72 h, and then labeled with annexin-V and propidium iodide (PI) for an apoptosis assay using flow cytometry. (B) Quantification of data in A. (C) The cells were treated as in A for analysis by a western blot assay for protein expression of apoptotic markers caspases-3 and -9 in CNE-1 and CNE-2 cells. GAPDH was used as an internal control. (D) Thiostrepton-treated cells were stained with Hoechst 33258, and their nuclear morphology was observed under an immunofluorescence microscope. (E) Quantification of nuclear morphological impairment shown in D. Quantitative data are expressed as the means±SD for individual groups of cells from 3 separate experiments. * P<0.05 compared to the controls. NPC – nasopharyngeal carcinoma; FoxM1 – forkhead box M1.