Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

16 December 2021: Animal Study

Forkhead Box Protein M1 Promotes Nasopharyngeal Carcinoma Cell Tumorigenesis Possibly via the Wnt/β-Catenin Signaling Pathway

Chao Yu 1ABEFG* , Hongyan Chen 2AEF , Yanli Zhao 1B , Yuedong Zhang 1BCD

DOI: 10.12659/MSM.931970

Med Sci Monit 2021; 27:e931970

Figure 2 Effect of inhibition of FoxM1 on NPC cell apoptosis and nuclear morphology(A) CNE-1 and CNE-2 cells were exposed to vehicle or 8 μM of thiostrepton for 72 h, and then labeled with annexin-V and propidium iodide (PI) for an apoptosis assay using flow cytometry. (B) Quantification of data in A. (C) The cells were treated as in A for analysis by a western blot assay for protein expression of apoptotic markers caspases-3 and -9 in CNE-1 and CNE-2 cells. GAPDH was used as an internal control. (D) Thiostrepton-treated cells were stained with Hoechst 33258, and their nuclear morphology was observed under an immunofluorescence microscope. (E) Quantification of nuclear morphological impairment shown in D. Quantitative data are expressed as the means±SD for individual groups of cells from 3 separate experiments. * P<0.05 compared to the controls. NPC – nasopharyngeal carcinoma; FoxM1 – forkhead box M1.

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750