Figure 5 Effect of inhibition of FoxM1 on Wnt/β-catenin signaling activity in NPC cells and NPC xenograft tissues(A) CNE-1 and CNE-2 cells were exposed to vehicle or 8 μM thiostrepton for 72 h, and then the protein expression of Wnt/β-catenin signaling-related proteins was evaluated by western blotting, as indicated. (B) Quantitative data for relative protein expression in CNE-1 cells. (C) Quantitative data for relative protein expression in CNE-2 cells. (D) At 42 days after intraperitoneal injection of vehicle or thiostrepton at a dose of 200 mg/kg body weight, the NPC xenografts were harvested. Tissue lysates were collected, and the protein expression of Wnt/β-catenin signaling-related proteins was evaluated by western blotting, as indicated. Protein expression levels were normalized to the expression level of GAPDH, and the results were then further quantified. (E) Quantitative data for relative protein expression in D. * P<0.05 compared to the control.