Figure 4 Sodium citrate (SC) does not affect anti-inflammatory, anti-catabolic, and regenerative protein enrichment. Protein concentrations of interleukin (IL)-1ra (A), platelet-derived growth factor (PDGF) (B), tissue inhibitor of metalloproteinase (TIMP)-1 (C), and TIMP-2 (D) in human blood samples are shown before incubation (1), after incubation at 37°C for 24 h in the presence of saline as a control (2), and after incubation at 37°C for 24 h in the presence of 4% SC (3). One-way analysis of variance (ANOVA) tests reveal that SC did not decrease the protein concentrations in 24-h incubated blood or in the final product (* P<0.01; *** P<0.0001; nonsignificant [ns], P>0.01). The figure was created using ImageJ software version 1.53e; Java 1.8.0_172.