Figure 5 Production of anti-inflammatory and anti-catabolic molecules in incubated blood samples from recovered COVID-19 patients. Blood samples from immunized (COVID-19 recovered) patients were incubated for 0, 6, or 24 h at 37°C with 4% sodium citrate (SC) and then analyzed for concentrations of interleukin (IL)-1ra (A), tissue inhibitor of metalloproteinase (TIMP)-1 (B), and TIMP-2 (C). IL-1ra (A) was significantly enriched starting after 6 h of incubation in 37°C (1-way ANOVA test). TIMP-1 was significantly enriched after 6 h of incubation (B), whereas TIMP-2 concentration remained unchanged (C) (1-way ANOVA test; *** P<0.0001; nonsignificant [ns], P>0.01). The figure was created using ImageJ software version 1.53e; Java 1.8.0_172.